【单选题】There are several isozymes of protein kinase C (PKC), each with a characteristic tissue distribution, target protein specificity, and role. Their targets include cytoskeletal proteins, enzymes, and __...
【判断题】During gene editing by CRISPR/Cas9, the genes encoding the Cas9 protein and sgRNA are introduced into a cell where a targeted genomic change is planned. The sgRNA has a region complementary to the cho...
【单选题】What is the advantage of using GFP-tag to visualize specific proteins, instead of staining cells with fluorescent dyes that bind to the target protein?
A.
GFP fusions enable one to track changes in the location of the protein in living cells; staining usually requires fixed cells
B.
GFP fusions enable higher resolution than staining with fluorescent probes.
C.
GFP permits the position of the protein in the cell more precisely than fluorescent probes
D.
GFP fusions are not subject to artifacts; fluorescent probes may introduce background artifacts.
【判断题】Signal molecule binds GPCR (G protein-coupled receptor) and causes conformational changes of the GPCR, which activates heterotrimeric G protein to further affect intracellular signaling proteins or ta...