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回答下列问题: ( 1 )博耶( H. Boyer) 和科恩(S. Coben) 将非洲爪蟾核糖体蛋白基因与质粒重组后导入大肠杆菌细胞中进行了表达 , 该研究除证明了质粒可以作为载体外 , 还证明了 _____( 答出两点即可) 。 ( 2 )体外重组的质粒可通过Ca 2+ 参与的 方法导入大肠杆菌细胞 : 而体外重组的噬菌体 DNA 通常需与 组装成完整噬菌体后 , 通过侵染的方法将重组的噬菌体 DNA 导入宿主细胞 , 在细菌、心肌细胞、叶肉细胞中 , 可作为重组噬菌体宿主细胞的是 。 ( 3 )真核生物基因( 目的基因) 在大肠杆菌细胞内表达时, 表达出的蛋白质可能会被降解。为防止蛋白质被降解 , 在实验中用 的大肠杆菌作为受体细胞 , 在蛋白质纯化的过程中应添加 的抑制剂。
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