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【单选题】
平板划线分离接种细菌时,下列要求中哪一项最关键( )。
A.
不能划破培养基
B.
培养后必须有明显的单个菌落出现
C.
必须有明显的分区划线
D.
培养后绝对无污染菌落出现
E.
最末区不能与原始区相连而形成的细菌集团
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