The principles of the Guide for the Care and Use of Laboratory Animals (8th Edition, 2011) are:
A.
Consideration of alternatives (in vitro systems, computer simulations, and/or mathematical models) to reduce or replace the use of animals
B.
Design and performance of procedures on the basis of relevance to human or animal health, advancement of knowledge, or the good of society
C.
Use of appropriate species, quality, and number of animals
D.
Avoidance or minimization of discomfort, distress, and pain