【单选题】By fusing the gene encoding a protein under study with the gene for an epitope tag, investigators can precipitate the protein product of the fusion gene by complexing it with the antibody that binds t...
【单选题】If fusion to green fluorescence protein (GFP) does not impair the function or properties of a protein one wishes to study, the fusion protein can be used to reveal the protein’s ________ in the cell u...
【单选题】The gene for almost any protein can be altered to express a fusion protein that can be purified by affinity chromatography. The peptide or protein used for this purpose is referred to as a tag. Which ...
A.
The gene encoding the target protein is fused to a gene encoding a peptide or protein that binds a large, unstable ligand with high affinity and specificity.
B.
Tag sequences can be added to genes such that the resulting proteins have tags at their amino or carboxyl terminus.
C.
Even very small tags can affect the properties of the proteins they are attached to, thereby influencing the study results.
D.
Even if the tag is removed by a protease, one or a few extra amino acid residues can remain behind on the target protein, which may or may not affect the protein’s activity.