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【单选题】
A mutant of E. coli with a heat-sensitive DNA ligase (25℃ permissive, 37℃ nonpermissive) has been used to show that DNA synthesis is discontinuous. Examination of DNA replication in the presence of [3H]-thymidine in the mutant would demonstrate which of following?A.
The accumulation of short segments of unlabeled DNA at 25 ℃ and at 37 ℃ .
B.
The accumulation of short segments of unlabeled DNA at 25 ℃ but not at 37 ℃ .
C.
The accumulation of short segments of radioactive DNA at 37 ℃ but not at 25 ℃.
D.
The incorporation of short fragments of radioactive DNA into longer ones at 25 ℃ and at 37 ℃ .
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【简答题】制造云是一种基于云制造内涵建立的智能制造系统,它是一种( )。
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