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【简答题】
你在做Southern印迹分析，并且刚完成了凝胶电泳这一步。根据方案，下面一个步骤是用NaOH溶液浸泡凝胶，使DNA变性为单链。为了节省时间，你略过了这一步，直接将DNA从凝胶转至硝酸纤维素膜上。然后用标记探针杂交，最后发现放射自显影片是空白。错在哪里?
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