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【单选题】
DNA的Tm值就是引起DNA变性的温度，对于一段DNA序列来说，下列哪个因素与其的Tm值高低无关？A.
DNA中GC的含量
B.
DNA可能形成的二级结构
C.
DNA所处的溶液环境（包括溶液pH值、离子强度、溶液组成等）
D.
测定Tm值所使用的仪器
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