【单选题】Please don't take ______ at what I'm saying.
【判断题】Don't take offence ——getting the form of adress "wrong" is rarely intended to be offensive.
【判断题】PCR产物具有特异性,是因为合适的变性、退火、延伸温度。()
【多选题】延伸温度应有如下特点 ( )
C.
Taq DNA 酶在延伸温度时有较高的酶活性
【判断题】Don't take offence—getting the form of address "wrong" is rarely intended to be offensive.
【简答题】PCR反应时,延伸温度一般为( ) °C ,退火温度一般比引物的Tm值要( )(填高或低)。
【单选题】PCR扩增DNA时,用于DNA延伸时的温度条件为
【判断题】PCR引物长度一般为15~30个核苷酸。过短影响PCR的特异性,过长会提高相应退火温度,使延伸温度超过TaqDNA聚合酶最适温度74℃,影响产物的生成。