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【简答题】
精子密度计数。 操作： 1）在显微镜的低倍镜下找到计算板的方格、然后调至高倍，找到某一个中方格，。 2）稀释精液，先用白细胞吸管吸取0.5-1.0处，然后吸取3%的氯NaCL液至刻度11，对精液进行10或20倍稀释。稀释中无论吸取精液还是NaCL液，液面只能缓缓上升，不能下降。吸取氯化钠后，用手指堵住吸管的两端，振荡几次，弃去精液2-3滴。 3）镜检：把稀释后的精液在与计算室之间滴一小滴，使精液自然充入，推动玻片，找到计算板上某一角的中方格，检查精子数目。一般检查5个中方格内的精子数，即四角和中央的一个中方格。 4）计算：检查完5个中方格的精子数以后，按下面公式计算：1ml原精液的精子数=5个中方格的精子数×5×10×1000×稀释倍数。 本实验要求做两次，如果两次结果接近。取其平均值。如果两次结果差别较大，再做第三次，其结果与前两次中哪一次的结果相近，即与该次精子密度取平均值，做为本次检查的最后结果。参考数据：精液精子的密度约为1-3亿/ml。 5）器械的洗涤：显微镜恢复原始状态、装箱。吸管、计算板等洗涤。擦净。为防止精子重复和漏掉，检查时要采取什么技术措施？
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