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【简答题】
说明钳工及其加工特点、基本操作工序、种类和应用。
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皮皮学刷刷变学霸
举一反三
【多选题】电泳的凝胶介质的高密度凝胶包括
A.
淀粉凝胶
B.
聚丙烯酰胺凝胶
C.
琼脂糖凝胶
D.
硅胶
【单选题】Which of the following enzymes cannot catalyze the formation of a phosphodiester bond?
A.
endonuclease
B.
RNA polymerase
C.
DNA polymerase
D.
ligase
E.
none of the choices are correct.
【单选题】The final phosphodiester bond between the fragments is made by .
A.
DNA ligase
B.
DNA pol Ⅲ
C.
DNA pol Ⅰ
D.
DNA primase
【多选题】下列( )属于烦恼产生的原因
A.
执着
B.
好高骛远
C.
太自私
D.
嫉妒心
【单选题】___________ restriction endonucleases, first isolated by Hamilton Smith in 1970, are simpler, require no ATP, and catalyze the hydrolytic cleavage of particular phosphodiester bonds in the DNA within ...
A.
Type I
B.
Type II
C.
Type III
D.
Type IV
【单选题】Which of the following would prevent the formation of a phosphodiester bond in DNA?
A.
removal of a nitrogen from one of the bases
B.
removal of oxygen from carbon 2 of the sugar
C.
removal of an oxygen from carbon 3 of the sugar or removal of the phosphate from the gamma position of the nucleoside triphosphate
D.
removal of the phosphate from the alpha position of the nucleoside triphosphate
E.
removal of the phosphate at the beta position of the nucleoside triphosphate
【单选题】Depurination does not break the DNA phosphodiester backbone but instead removes a purine base from a nucleotide, giving rise to lesions that resemble missing _____.
A.
nose
B.
eyes
C.
teeth
D.
ears
【单选题】随着变换反应的进行,变换率增大,最适宜温度()。
A.
降低
B.
升高
C.
不变
D.
没有关系
【单选题】DNA ligase catalyzes the formation of a phosphodiester bond between a 3′ hydroxyl at the end of one DNA strand and a 5′ phosphate at the end of another strand. The phosphate must be activated by adeny...
A.
AMP
B.
ADP
C.
NAD +
D.
NADP +
【单选题】Once the two fragments are joined, the final sealing phosphodiester bond is formed by
A.
Polymerase
B.
exonuclease
C.
Transcriptase
D.
Ligase
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