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在一根 3m 长得色谱柱上分离两个组分,得到数据: t M =1min , t R1 =14 min , t R2 =17min , W 2 =1 min ,求: ( 1 )求调整保留时间 t’ R1 及 t’ R2 ( 2 )用组分 2 计算色谱柱的理论塔板数; ( 3 )若达到分辨率 R=1.5 ,所需的最短柱长是几米?
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