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【简答题】
有一个A肽,经酸解分析得知为Lys、His、Asp、Glu2、Ala以及Val、Tyr和两个NH3分子组成。当A肽与FDNB试剂反应后得DNP-Asp;当用羧肽酶处理后得游离缬氨酸。如果我们在实验中将A肽用胰蛋白酶降解时,得到两种肽,其中一种(Lys、Asp、Glu、Ala、Tyr)在pH6.4时,净电荷为零,另一种(His、Glu以及Val)可给除DNP-His,在pH6.4时,带正电荷。此外,A肽用蛋白酶降解时,也得到两种肽,其中一种(Asp、Ala、Tyr)在pH6.4时全中性,另一种(Lys、His、Glu2以及Val)在pH6.4时带正电荷。问A肽的氨基酸序列如
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